The show opened with Fallon and his Co-Host Steve Higgins entering Diagon Alley. There was a quick pan of Gringott's Bank. Higgins tried to use the Firebolt broom but slammed into a building. Higgins let Fallon go ahead with his journey. Fallon boarded the Hogwarts Express to get to the Tonight Show taping. We saw the streamrolling train in action departing from King's Cross Station. Upon reaching its destination, Fallon hopped off the train and was handfived by students dressed in robes. Running down a red carpet Fallon met all kinds of popular Universal characters including the Despicable Me minions and The Simpsons. Eventually he reached Studio 20 located on the Universal Studios backlot.
Following the monologue Fallon exclaimed the amazing lands that Universal Studios has to offer. He declared that he has "seen the future of theme parks and it is Diagon Alley".
After a commercial break Fallon introduced a clip where he and Higgins had traveled to Ollivander's Wand Shop to get an authentic Harry Potter wand. The two of them exchanged mature wand jokes such as the size of the wand for Fallon being very small. Their wands eventually chose the wizards after Fallon made a vase of flowers wilt, and Higgins made them bloom again. Upon leaving the famous theme from The Breakfast Club "Don't You Forget About Me" played as Fallon and Higgins emotionally left Ollivanders with wands in hand.
The rest of the evening taping included an edited rap of NBC News Anchor rapping Sir Mix A Lot's Baby Got Back, and interviews with the in studio guests Keenen Ivory Wayans and musical guest Jennifer Lopez.